Determination of triamcinolone acetonide in silicone oil and aqueous humor of vitrectomized rabbits’ eyes:

Determination of triamcinolone acetonide in silicone oil and aqueous humor of vitrectomized rabbits’ eyes: Application for a pharmacokinetic study with intravitreal triamcinolone acetonide injections (Kenalog® 40).

Gabriella M. Fernandes-Cunha, Juliana B. Saliba, Rubens C. Siqueira, Rodrigo Jorge, Armando Silva-Cunha.

Journal of Pharmaceutical and Biomedical Analysis,

Volume 89,

2014,

Pages 24-27,

ISSN 0731-7085,

https://doi.org/10.1016/j.jpba.2013.10.025.

(http://www.sciencedirect.com/science/article/pii/S0731708513004846)

Abstract A simple and accurate method including liquid–liquid extraction and protein precipitation procedures from silicone oil and aqueous humor samples followed by high-performance liquid chromatography (HPLC-UV) was developed and validated to determine the pharmacokinetic profile of triamcinolone acetonide in silicone oil and aqueous humor of rabbits’ eyes submitted to the pars plana vitrectomy surgery. The method was successfully applied to quantify the drug remaining in silicone oil and aqueous humor (LOQ range of 1 μg/mL). The triamcinolone acetonide remained in silicone oil and aqueous humor of vitrectomized rabbits’ eyes for four weeks after the intravitreal injections.

Graphical abstract

Highlights

•High-performance liquid chromatography method was developed and validated.•Administration of TA injections in rabbits’ eyes.•Method was successfully applied to quantify the drug in SO and aqueous humor.•TA remained in SO and aqueous humor of rabbits’ eyes for 4 weeks.•SO may play an important role in the elimination of lipophilic drugs.

Gabriella M. Fernandes-Cunha, Juliana B. Saliba, Rubens C. Siqueira, Rodrigo Jorge, Armando Silva-Cunha,